Establishment of a Novel Anti-Mouse CCR1 Monoclonal Antibody C1Mab-6.
Monoclon Antib Immunodiagn Immunother
; 43(2): 67-74, 2024 Apr.
Article
en En
| MEDLINE
| ID: mdl-38512465
ABSTRACT
C-C motif chemokine receptor 1 (CCR1/CD191) is a member of G-protein-coupled receptors and is expressed on myeloid cells, such as neutrophils and macrophages. Because the CCR1 signaling promotes tumor expansion in the tumor microenvironment (TME), the modification of TME is an effective strategy for cancer therapy. Although CCR1 is an attractive target for solid tumors and hematological malignancies, therapeutic agents for CCR1 have not been approved. Here, we established a novel anti-mouse CCR1 (mCCR1) monoclonal antibody (mAb), C1Mab-6 (rat IgG2b, kappa), using the Cell-Based Immunization and Screening method. Flow cytometry and Western blot analyses showed that C1Mab-6 recognizes mCCR1 specifically. The dissociation constant of C1Mab-6 for mCCR1-overexpressed Chinese hamster ovary-K1 was determined as 3.9 × 10-9 M, indicating that C1Mab-6 possesses a high affinity to mCCR1. These results suggest that C1Mab-6 could be a useful tool for targeting mCCR1 in preclinical mouse models.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Macrófagos
/
Anticuerpos Monoclonales
Límite:
Animals
Idioma:
En
Revista:
Monoclon Antib Immunodiagn Immunother
Año:
2024
Tipo del documento:
Article
País de afiliación:
Japón