Single-cell transcriptomic analysis of radiation-induced lung injury in rat.
Biomol Biomed
; 24(5): 1331-1349, 2024 Sep 06.
Article
en En
| MEDLINE
| ID: mdl-38552230
ABSTRACT
Radiation-induced lung injury (RILI) frequently occurs as a complication following radiotherapy for chest tumors like lung and breast cancers. However, the precise underlying mechanisms of RILI remain unclear. In this study, we generated RILI models in rats treated with a single dose of 20 Gy and examined lung tissues by single-cell RNA sequencing (scRNA-seq) 2 weeks post-radiation. Analysis of lung tissues revealed 18 major cell populations, indicating an increase in cell-cell communication following radiation exposure. Neutrophils, macrophages, and monocytes displayed distinct subpopulations and uncovered potential for pro-inflammatory effects. Additionally, endothelial cells exhibited a highly inflammatory profile and the potential for reactive oxygen species (ROS) production. Furthermore, smooth muscle cells (SMC) showed a high propensity for extracellular matrix (ECM) deposition. Our findings broaden the current understanding of RILI and highlight potential avenues for further investigation and clinical applications.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Lesión Pulmonar
/
Análisis de la Célula Individual
Límite:
Animals
Idioma:
En
Revista:
Biomol Biomed
Año:
2024
Tipo del documento:
Article
País de afiliación:
China