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Selective-plane-activation structured illumination microscopy.
Temma, Kenta; Oketani, Ryosuke; Kubo, Toshiki; Bando, Kazuki; Maeda, Shunsuke; Sugiura, Kazunori; Matsuda, Tomoki; Heintzmann, Rainer; Kaminishi, Tatsuya; Fukuda, Koki; Hamasaki, Maho; Nagai, Takeharu; Fujita, Katsumasa.
Afiliación
  • Temma K; Department of Applied Physics, Osaka University, Osaka, Japan.
  • Oketani R; Advanced Photonics and Biosensing Open Innovation Laboratory, AIST-Osaka University, Osaka, Japan.
  • Kubo T; Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Osaka, Japan.
  • Bando K; Department of Applied Physics, Osaka University, Osaka, Japan.
  • Maeda S; Department of Chemistry, Kyushu University, Fukuoka, Japan.
  • Sugiura K; Department of Applied Physics, Osaka University, Osaka, Japan.
  • Matsuda T; Department of Applied Physics, Osaka University, Osaka, Japan.
  • Heintzmann R; Department of Applied Physics, Osaka University, Osaka, Japan.
  • Kaminishi T; SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Osaka, Japan.
  • Fukuda K; SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Osaka, Japan.
  • Hamasaki M; Leibniz Institute of Photonic Technology, Jena, Germany.
  • Nagai T; Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller University Jena, Jena, Germany.
  • Fujita K; Institute for Open and Transdisciplinary Research Initiatives, Osaka University, Osaka, Japan.
Nat Methods ; 21(5): 889-896, 2024 May.
Article en En | MEDLINE | ID: mdl-38580844
ABSTRACT
The background light from out-of-focus planes hinders resolution enhancement in structured illumination microscopy when observing volumetric samples. Here we used selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. Theoretical investigation of the imaging properties and experimental demonstrations show that selective plane activation is beneficial for imaging dense microstructures in cells and cell spheroids.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microscopía Fluorescente Límite: Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2024 Tipo del documento: Article País de afiliación: Japón
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microscopía Fluorescente Límite: Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2024 Tipo del documento: Article País de afiliación: Japón