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SPTLC3 Is Essential for Complex I Activity and Contributes to Ischemic Cardiomyopathy.
Kovilakath, Anna; Mauro, Adolfo G; Valentine, Yolander A; Raucci, Frank J; Jamil, Maryam; Carter, Christiane; Thompson, Jeremy; Chen, Qun; Beutner, Gisela; Yue, Yang; Allegood, Jeremy; Wang, Xiaoxin X; Dail, Jordan; Devarakonda, Teja; Myakala, Komuraiah; Windle, Jolene J; Subler, Mark A; Montefusco, David; Willard, Belinda; Javaheri, Ali; Bernas, Tytus; Mahata, Sushil K; Levi, Moshe; Liu, Jinze; Porter, George A; Lesnefsky, Edward J; Salloum, Fadi N; Cowart, L Ashley.
Afiliación
  • Kovilakath A; Department of Human and Molecular Genetics (A.K., M.J., J.J.W., M.A.S.), Virginia Commonwealth University, Richmond.
  • Mauro AG; Department of Internal Medicine, Division of Cardiology, Pauley Heart Center, Richmond, VA (A.G.M., J.T., Q.C., T.D., E.J.L., F.N.S.).
  • Valentine YA; C. Kenneth and Dianne Wright Center for Clinical and Translational Research (Y.A.V.), Virginia Commonwealth University, Richmond.
  • Raucci FJ; Department of Biochemistry and Molecular Biology (Y.A.V., Y.Y., J.A., J.D., D.M., E.J.L., L.A.C.), Virginia Commonwealth University, Richmond.
  • Jamil M; Department of Pediatrics, Division of Pediatric Cardiology (F.J.R.), Virginia Commonwealth University, Richmond.
  • Carter C; Department of Human and Molecular Genetics (A.K., M.J., J.J.W., M.A.S.), Virginia Commonwealth University, Richmond.
  • Thompson J; Bioinformatics Shared Resource, Massey Comprehensive Cancer Center (C.C., J.L.), Virginia Commonwealth University, Richmond.
  • Chen Q; Department of Internal Medicine, Division of Cardiology, Pauley Heart Center, Richmond, VA (A.G.M., J.T., Q.C., T.D., E.J.L., F.N.S.).
  • Beutner G; Department of Internal Medicine, Division of Cardiology, Pauley Heart Center, Richmond, VA (A.G.M., J.T., Q.C., T.D., E.J.L., F.N.S.).
  • Yue Y; Department of Pediatrics (G.B., G.A.P.), University of Rochester Medical Center, NY.
  • Allegood J; Department of Biochemistry and Molecular Biology (Y.A.V., Y.Y., J.A., J.D., D.M., E.J.L., L.A.C.), Virginia Commonwealth University, Richmond.
  • Wang XX; Department of Biochemistry and Molecular Biology (Y.A.V., Y.Y., J.A., J.D., D.M., E.J.L., L.A.C.), Virginia Commonwealth University, Richmond.
  • Dail J; Department of Biochemistry and Molecular & Cellular Biology, Georgetown University, Washington, DC (X.X.W., K.M., M.L.).
  • Devarakonda T; Department of Biochemistry and Molecular Biology (Y.A.V., Y.Y., J.A., J.D., D.M., E.J.L., L.A.C.), Virginia Commonwealth University, Richmond.
  • Myakala K; Department of Physiology and Biophysics (F.N.S., T.D., E.J.L.), Virginia Commonwealth University, Richmond.
  • Windle JJ; Department of Internal Medicine, Division of Cardiology, Pauley Heart Center, Richmond, VA (A.G.M., J.T., Q.C., T.D., E.J.L., F.N.S.).
  • Subler MA; Department of Biochemistry and Molecular & Cellular Biology, Georgetown University, Washington, DC (X.X.W., K.M., M.L.).
  • Montefusco D; Department of Human and Molecular Genetics (A.K., M.J., J.J.W., M.A.S.), Virginia Commonwealth University, Richmond.
  • Willard B; Massey Comprehensive Cancer Center (J.J.W., J.L., F.N.S., L.A.C.), Virginia Commonwealth University, Richmond.
  • Javaheri A; Department of Human and Molecular Genetics (A.K., M.J., J.J.W., M.A.S.), Virginia Commonwealth University, Richmond.
  • Bernas T; Department of Biochemistry and Molecular Biology (Y.A.V., Y.Y., J.A., J.D., D.M., E.J.L., L.A.C.), Virginia Commonwealth University, Richmond.
  • Mahata SK; Proteomics and Metabolomics Shared Laboratory Resource, Lerner Research Institute, Cleveland Clinic, OH (B.W.).
  • Levi M; Cardiovascular Division, Department of Medicine, Washington University School of Medicine, St. Louis, MO (A.J.).
  • Liu J; St. Louis Veterans' Affairs Medical Center, MO (A.J.).
  • Porter GA; Department of Anatomy and Neurobiology (T.B.), Virginia Commonwealth University, Richmond.
  • Lesnefsky EJ; Veterans' Affairs San Diego Healthcare System and University of California San Diego, (S.K.M).
  • Salloum FN; Department of Biochemistry and Molecular & Cellular Biology, Georgetown University, Washington, DC (X.X.W., K.M., M.L.).
  • Cowart LA; Bioinformatics Shared Resource, Massey Comprehensive Cancer Center (C.C., J.L.), Virginia Commonwealth University, Richmond.
Circulation ; 150(8): 622-641, 2024 Aug 20.
Article en En | MEDLINE | ID: mdl-38660786
ABSTRACT

BACKGROUND:

Dysregulated metabolism of bioactive sphingolipids, including ceramides and sphingosine-1-phosphate, has been implicated in cardiovascular disease, although the specific species, disease contexts, and cellular roles are not completely understood. Sphingolipids are produced by the serine palmitoyltransferase enzyme, canonically composed of 2 subunits, SPTLC1 (serine palmitoyltransferase long chain base subunit 1) and SPTLC2 (serine palmitoyltransferase long chain base subunit 2). Noncanonical sphingolipids are produced by a more recently described subunit, SPTLC3 (serine palmitoyltransferase long chain base subunit 3).

METHODS:

The noncanonical (d16) and canonical (d18) sphingolipidome profiles in cardiac tissues of patients with end-stage ischemic cardiomyopathy and in mice with ischemic cardiomyopathy were analyzed by targeted lipidomics. Regulation of SPTLC3 by HIF1α under ischemic conditions was determined with chromatin immunoprecipitation. Transcriptomics, lipidomics, metabolomics, echocardiography, mitochondrial electron transport chain, mitochondrial membrane fluidity, and mitochondrial membrane potential were assessed in the cSPTLC3KO transgenic mice we generated. Furthermore, morphological and functional studies were performed on cSPTLC3KO mice subjected to permanent nonreperfused myocardial infarction.

RESULTS:

Herein, we report that SPTLC3 is induced in both human and mouse models of ischemic cardiomyopathy and leads to production of atypical sphingolipids bearing 16-carbon sphingoid bases, resulting in broad changes in cell sphingolipid composition. This induction is in part attributable to transcriptional regulation by HIF1α under ischemic conditions. Furthermore, cardiomyocyte-specific depletion of SPTLC3 in mice attenuates oxidative stress, fibrosis, and hypertrophy in chronic ischemia, and mice demonstrate improved cardiac function and increased survival along with increased ketone and glucose substrate metabolism utilization. Depletion of SPTLC3 mechanistically alters the membrane environment and subunit composition of mitochondrial complex I of the electron transport chain, decreasing its activity.

CONCLUSIONS:

Our findings suggest a novel essential role for SPTLC3 in electron transport chain function and a contribution to ischemic injury by regulating complex I activity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Complejo I de Transporte de Electrón / Serina C-Palmitoiltransferasa / Cardiomiopatías Límite: Animals / Humans / Male Idioma: En Revista: Circulation Año: 2024 Tipo del documento: Article
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Complejo I de Transporte de Electrón / Serina C-Palmitoiltransferasa / Cardiomiopatías Límite: Animals / Humans / Male Idioma: En Revista: Circulation Año: 2024 Tipo del documento: Article