Sortase-Modified Cholera Toxoids Show Specific Golgi Localization.
Toxins (Basel)
; 16(4)2024 Apr 16.
Article
en En
| MEDLINE
| ID: mdl-38668619
ABSTRACT
Cholera toxoid is an established tool for use in cellular tracing in neuroscience and cell biology. We use a sortase labeling approach to generate site-specific N-terminally modified variants of both the A2-B5 heterohexamer and B5 pentamer forms of the toxoid. Both forms of the toxoid are endocytosed by GM1-positive mammalian cells, and while the heterohexameric toxoid was principally localized in the ER, the B5 pentamer showed an unexpectedly specific localization in the medial/trans-Golgi. This study suggests a future role for specifically labeled cholera toxoids in live-cell imaging beyond their current applications in neuronal tracing and labeling of lipid rafts in fixed cells.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cisteína Endopeptidasas
/
Toxina del Cólera
/
Aparato de Golgi
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Toxins (Basel)
Año:
2024
Tipo del documento:
Article
País de afiliación:
Reino Unido