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Protocol for the isolation of the mouse sympathetic splanchnic-celiac-superior mesenteric ganglion complex.
Trevizan-Baú, Pedro; Ringuet, Mitchell Ty; Stebbing, Martin J; McAllen, Robin M; Furness, John B; Mueller, Scott N.
Afiliación
  • Trevizan-Baú P; The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, VIC, Australia; Department of Physiological Sciences, University of Florida, Gainesvill
  • Ringuet MT; The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, VIC, Australia.
  • Stebbing MJ; The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia.
  • McAllen RM; The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia.
  • Furness JB; The Florey Institute, The University of Melbourne, Melbourne, VIC, Australia; Department of Anatomy and Physiology, University of Melbourne, Parkville, VIC, Australia.
  • Mueller SN; Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, VIC, Australia. Electronic address: smue@unimelb.edu.au.
STAR Protoc ; 5(2): 103036, 2024 Jun 21.
Article en En | MEDLINE | ID: mdl-38676927
ABSTRACT
Neurons that originate from pre-vertebral sympathetic ganglia, the splanchnic-celiac-superior mesenteric ganglion complex (SCSMG) in mouse, have important roles in control of organs of the upper abdomen. Here, we present a protocol for the isolation of the mouse sympathetic SCSMG. We describe steps for surgical incision, ganglia isolation, ganglia fine dissection, and whole-mount SCSMG after clearing-enhanced 3D (Ce3D) clearing method and immunohistochemistry. Given the importance of mice in studies of that control, this protocol aims to assist biomedical researchers in the dissection of the mouse SCSMG.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervios Esplácnicos / Ganglios Simpáticos Límite: Animals Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nervios Esplácnicos / Ganglios Simpáticos Límite: Animals Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article