Development of an effective single-chain variable fragment recognizing a novel epitope in the hepatitis C virus E2 protein that restricts virus entry into hepatocytes.
Arch Virol
; 169(5): 112, 2024 Apr 29.
Article
en En
| MEDLINE
| ID: mdl-38683226
ABSTRACT
Previously, we reported a neutralizing monoclonal antibody, A8A11, raised against a novel conserved epitope within the hepatitis C virus (HCV) E2 protein, that could significantly reduce HCV replication. Here, we report the nucleotide sequence of A8A11 and demonstrate the efficacy of a single-chain variable fragment (scFv) protein that mimics the antibody, inhibits the binding of an HCV virus-like particle to hepatocytes, and reduces viral RNA replication in a cell culture system. More importantly, scFv A8A11 was found to effectively restrict the increase of viral RNA levels in the serum of HCV-infected chimeric mice harbouring human hepatocytes. These results suggest a promising approach to neutralizing-antibody-based therapeutic interventions against HCV infection.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas del Envoltorio Viral
/
Hepacivirus
/
Hepatocitos
/
Internalización del Virus
/
Anticuerpos de Cadena Única
/
Epítopos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Arch Virol
Año:
2024
Tipo del documento:
Article
País de afiliación:
India