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Storage conditions affect the composition of the lyophilized secretome of multipotent mesenchymal stromal cells.
Rogulska, Olena; Vackova, Irena; Prazak, Simon; Turnovcova, Karolina; Kubinova, Sarka; Bacakova, Lucie; Jendelova, Pavla; Petrenko, Yuriy.
Afiliación
  • Rogulska O; Laboratory of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200, Prague, Czech Republic.
  • Vackova I; Department of Neuroregeneration, Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, 14200, Prague, Czech Republic.
  • Prazak S; Laboratory of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200, Prague, Czech Republic. irena.vackova@fgu.cas.cz.
  • Turnovcova K; Laboratory of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200, Prague, Czech Republic.
  • Kubinova S; Department of Neuroregeneration, Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, 14200, Prague, Czech Republic.
  • Bacakova L; Department of Optical and Biophysical Systems, Institute of Physics of the Czech Academy of Sciences, Na Slovance 1999/2, 18221, Prague, Czech Republic.
  • Jendelova P; Laboratory of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200, Prague, Czech Republic.
  • Petrenko Y; Department of Neuroregeneration, Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, 14200, Prague, Czech Republic.
Sci Rep ; 14(1): 10243, 2024 05 03.
Article en En | MEDLINE | ID: mdl-38702388
ABSTRACT
The widespread use of multipotent mesenchymal stromal cell-derived secretome (MSC-sec) requires optimal preservation methods. Lyophilization offers benefits like concentrating the secretome, reducing the storage volume, and making storage conditions more flexible. This study evaluated the influence of storage duration and temperature on lyophilized MSC-sec. The conditioned medium from Wharton's jelly MSCs was stored at - 80 °C or lyophilized with or without trehalose. Lyophilized formulations were kept at - 80 °C, - 20 °C, 4 °C, or room temperature (RT) for 3 and 30 months. After storage and reconstitution, the levels of growth factors and cytokines were assessed using multiplex assay. The storage of lyophilized MSC-sec at - 80 °C ensured biomolecule preservation for 3 and 30 months. Following 3 month storage at 4 °C and RT, a notable decrease occurred in BDNF, bNGF, and sVCAM-1 levels. Prolonged 30 month storage at the same temperatures significantly reduced BDNF, bNGF, VEGF-A, IL-6, and sVCAM-1, while storage at - 20 °C decreased BDNF, bNGF, and VEGF- A levels. Trehalose supplementation of MSC-sec improved the outcome during storage at 4 °C and RT. Proper storage conditions were crucial for the preservation of lyophilized MSC-sec composition. Short-term storage at various temperatures maintained over 60% of the studied growth factors and cytokines; long-term preservation was only adequate at -80 °C.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Mesenquimatosas / Liofilización Límite: Humans Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: República Checa

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Mesenquimatosas / Liofilización Límite: Humans Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: República Checa
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