Shewanella phaeophyticola sp. nov. and Vibrio algarum sp. nov., isolated from marine brown algae.
Int J Syst Evol Microbiol
; 74(5)2024 May.
Article
en En
| MEDLINE
| ID: mdl-38728177
ABSTRACT
Two Gram-stain-negative, rod-shaped bacteria, designated as strains KJ10-1T and KJ40-1T, were isolated from marine brown algae. Both strains were catalase-positive, oxidase-positive, and facultative aerobic. Strain KJ10-1T exhibited optimal growth at 25â°C, pH 7.0, and 3â% NaCl, whereas strain KJ40-1T showed optimal growth at 25â°C, pH 7.0, and 2â% NaCl. The respiratory quinones of strain KJ10-1T were ubiquinone-8, ubiquinone-7, menaquinone-7, and methylated menaquinone-7, while the respiratory quinone of strain KJ40-1T was only ubiquinone-8. As major fatty acids, strain KJ10-1T contained C16ââ0, C17ââ1 ω8c, iso-C15ââ0, and summed feature 3 (C16ââ1 ω7c and/or C16ââ1 ω6c) and strain KJ40-1T contained C16ââ0 and summed features 3 and 8 (C18ââ1 ω7c and/or C18ââ1 ω6c). The major polar lipids in strain KJ10-1T were phosphatidylethanolamine, phosphatidylglycerol, and an unidentified aminolipid, whereas those in strain KJ40-1T were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The DNA G+C contents of strains KJ10-1T and KJ40-1T were 42.1 and 40.8âmol%, respectively. Based on 16S rRNA gene sequences, strains KJ10-1T and KJ40-1T exhibited the closest relatedness to Shewanella saliphila MMS16-UL250T (98.6â%) and Vibrio rumoiensis S-1T (95.4â%), respectively. Phylogenetic analyses, based on both 16S rRNA and 92 housekeeping genes, showed that the strains formed distinct phylogenic lineages within the genera Shewanella and Vibrio. Digital DNA-DNA hybridization and orthologous average nucleotide identity values between strain KJ10-1T and other Shewanella species, as well as between strain KJ40-1T and other Vibrio species, were below the thresholds commonly accepted for prokaryotic species delineation. Based on the phenotypic, chemotaxonomic, and phylogenetic data, strains KJ10-1T and KJ40-1T represent novel species of the genera Shewanella and Vibrio, respectively, for which the names Shewanella phaeophyticola sp. nov. and Vibrio algarum sp. nov. are proposed, respectively. The type strains of S. phaeophyticola and V. algarum are KJ10-1T (=KACC 22589T=JCM 35409T) and KJ40-1T (=KACC 22588T=JCM 35410T), respectively.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Filogenia
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Composición de Base
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Vibrio
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ADN Bacteriano
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ARN Ribosómico 16S
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Técnicas de Tipificación Bacteriana
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Ubiquinona
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Análisis de Secuencia de ADN
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Shewanella
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Vitamina K 2
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2024
Tipo del documento:
Article