Massively parallel CRISPR-assisted homologous recombination enables saturation editing of full-length endogenous genes in yeast.

Deng, Lei; Zhou, Yi-Lian; Cai, Zhenkun; Zhu, Jie; Li, Zenan; Bao, Zehua

Deng, Lei; Zhou, Yi-Lian; Cai, Zhenkun; Zhu, Jie; Li, Zenan; Bao, Zehua.

Afiliación

Deng L; Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310058, Zhejiang, China.
Zhou YL; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou 311215, Zhejiang, China.
Cai Z; Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310058, Zhejiang, China.
Zhu J; Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310058, Zhejiang, China.
Li Z; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou 311215, Zhejiang, China.
Bao Z; Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310058, Zhejiang, China.

Sci Adv ; 10(20): eadj9382, 2024 May 17.

Article en En | MEDLINE | ID: mdl-38748797

Asunto(s)

Sistemas CRISPR-Cas; Edición Génica; Recombinación Homóloga; Saccharomyces cerevisiae; Edición Génica/métodos; Saccharomyces cerevisiae/genética; Codón/genética; Genoma Fúngico

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Recombinación Homóloga / Sistemas CRISPR-Cas / Edición Génica Idioma: En Revista: Sci Adv Año: 2024 Tipo del documento: Article País de afiliación: China

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