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Fibroblast growth factor pathway promotes glycolysis by activating LDHA and suppressing LDHB in a STAT1-dependent manner in prostate cancer.
Ye, Yongkang; Yang, Fukan; Gu, Zhanhao; Li, Wenxuan; Yuan, Yinjiao; Liu, Shaoqian; Zhou, Le; Han, Bo; Zheng, Ruinian; Cao, Zhengguo.
Afiliación
  • Ye Y; Department of Urology, The Tenth Affiliated Hospital of Southern Medical University (Dongguan people's hospital), 523059, Dongguan, China.
  • Yang F; Department of Urology, Guangdong Medical University, Graduate School, 524002, Zhanjiang, China.
  • Gu Z; Department of Urology, Guangdong Medical University, Graduate School, 524002, Zhanjiang, China.
  • Li W; Department of Oncology, Dongguan Institute of Clinical Cancer Research, Dongguan Key Laboratory of Precision Diagnosis and Treatment for Tumors, The Tenth Affiliated Hospital of Southern Medical University (Dongguan people's hospital), 523059, Dongguan, China.
  • Yuan Y; Department of Oncology, Dongguan Institute of Clinical Cancer Research, Dongguan Key Laboratory of Precision Diagnosis and Treatment for Tumors, The Tenth Affiliated Hospital of Southern Medical University (Dongguan people's hospital), 523059, Dongguan, China.
  • Liu S; The First School of Clinical Medicine, Southern Medical University, 510510, Guangzhou, China.
  • Zhou L; Department of Urology, The Tenth Affiliated Hospital of Southern Medical University (Dongguan people's hospital), 523059, Dongguan, China.
  • Han B; Department of Oncology, Dongguan Institute of Clinical Cancer Research, Dongguan Key Laboratory of Precision Diagnosis and Treatment for Tumors, The Tenth Affiliated Hospital of Southern Medical University (Dongguan people's hospital), 523059, Dongguan, China.
  • Zheng R; The First School of Clinical Medicine, Southern Medical University, 510510, Guangzhou, China.
  • Cao Z; Department of Oncology, Dongguan Institute of Clinical Cancer Research, Dongguan Key Laboratory of Precision Diagnosis and Treatment for Tumors, The Tenth Affiliated Hospital of Southern Medical University (Dongguan people's hospital), 523059, Dongguan, China.
J Transl Med ; 22(1): 474, 2024 May 19.
Article en En | MEDLINE | ID: mdl-38764020
ABSTRACT

BACKGROUND:

The initiation of fibroblast growth factor 1 (FGF1) expression coincident with the decrease of FGF2 expression is a well-documented event in prostate cancer (PCa) progression. Lactate dehydrogenase A (LDHA) and LDHB are essential metabolic products that promote tumor growth. However, the relationship between FGF1/FGF2 and LDHA/B-mediated glycolysis in PCa progression is not reported. Thus, we aimed to explore whether FGF1/2 could regulate LDHA and LDHB to promote glycolysis and explored the involved signaling pathway in PCa progression.

METHODS:

In vitro studies used RT‒qPCR, Western blot, CCK-8 assays, and flow cytometry to analyze gene and protein expression, cell viability, apoptosis, and cell cycle in PCa cell lines. Glycolysis was assessed by measuring glucose consumption, lactate production, and extracellular acidification rate (ECAR). For in vivo studies, a xenograft mouse model of PCa was established and treated with an FGF pathway inhibitor, and tumor growth was monitored.

RESULTS:

FGF1, FGF2, and LDHA were expressed at high levels in PCa cells, while LDHB expression was low. FGF1/2 positively modulated LDHA and negatively modulated LDHB in PCa cells. The depletion of FGF1, FGF2, or LDHA reduced cell proliferation, induced cell cycle arrest, and inhibited glycolysis. LDHB overexpression showed similar inhibitory effect on PCa cells. Mechanistically, we found that FGF1/2 positively regulated STAT1 and STAT1 transcriptionally activated LDHA expression while suppressed LDHB expression. Furthermore, the treatment of an FGF pathway inhibitor suppressed PCa tumor growth in mice.

CONCLUSION:

The FGF pathway facilitates glycolysis by activating LDHA and suppressing LDHB in a STAT1-dependent manner in PCa.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Próstata / Transducción de Señal / Factor de Transcripción STAT1 / Factores de Crecimiento de Fibroblastos / Glucólisis / L-Lactato Deshidrogenasa Límite: Animals / Humans / Male Idioma: En Revista: J Transl Med Año: 2024 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Próstata / Transducción de Señal / Factor de Transcripción STAT1 / Factores de Crecimiento de Fibroblastos / Glucólisis / L-Lactato Deshidrogenasa Límite: Animals / Humans / Male Idioma: En Revista: J Transl Med Año: 2024 Tipo del documento: Article País de afiliación: China
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