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Establishment of acquired tracheoesophageal fistula using a modified magnetic compression technique in rabbits and its postmodeling evaluation.
Meng, Han; Nan, Fu-Yao; Kou, Na; Hong, Qin-Yan; Lv, Ming-Sheng; Li, Ju-Bo; Zhang, Bao-Jie; Zou, Hang; Li, Lei; Wang, Hong-Wu.
Afiliación
  • Meng H; Respiratory Disease Center, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100029, China.
  • Nan FY; The First Clinical Medical College, Beijing University of Chinese Medicine, Beijing 100700, China.
  • Kou N; Respiratory Disease Center, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100029, China.
  • Hong QY; The First Clinical Medical College, Beijing University of Chinese Medicine, Beijing 100700, China.
  • Lv MS; Respiratory Disease Center, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100029, China.
  • Li JB; Respiratory Disease Center, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100029, China.
  • Zhang BJ; The First Clinical Medical College, Beijing University of Chinese Medicine, Beijing 100700, China.
  • Zou H; Respiratory Disease Center, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100029, China.
  • Li L; Department of Animal Experimental Center, National Center for Cardiovascular, Fuwai Hospital, Beijing 100037, China.
  • Wang HW; Department of Animal Experimental Center, National Center for Cardiovascular, Fuwai Hospital, Beijing 100037, China.
World J Gastrointest Surg ; 16(5): 1385-1394, 2024 May 27.
Article en En | MEDLINE | ID: mdl-38817293
ABSTRACT

BACKGROUND:

Previous studies have validated the efficacy of both magnetic compression and surgical techniques in creating rabbit tracheoesophageal fistula (TEF) models. Magnetic compression achieves a 100% success rate but requires more time, while surgery, though less frequently successful, offers rapid model establishment and technical maturity in larger animal models.

AIM:

To determine the optimal approach for rabbit disease modeling and refine the process.

METHODS:

TEF models were created in 12 rabbits using both the modified magnetic compression technique and surgery. Comparisons of the time to model establishment, success rate, food and water intake, weight changes, activity levels, bronchoscopy findings, white blood cell counts, and biopsies were performed. In response to the failures encountered during modified magnetic compression modeling, we increased the sample size to 15 rabbit models and assessed the repeatability and stability of the models, comparing them with the original magnetic compression technique.

RESULTS:

The modified magnetic compression technique achieved a 66.7% success rate, whereas the success rate of the surgery technique was 33.3%. Surviving surgical rabbits might not meet subsequent experimental requirements due to TEF-related inflammation. In the modified magnetic compression group, one rabbit died, possibly due to magnet corrosion, and another died from tracheal magnet obstruction. Similar events occurred during the second round of modified magnetic compression modeling, with one rabbit possibly succumbing to aggravated lung infection. The operation time of the first round of modified magnetic compression was 3.2 ± 0.6 min, which was significantly reduced to 2.1 ± 0.4 min in the second round, compared to both the first round and that of the original technique.

CONCLUSION:

The modified magnetic compression technique exhibits lower stress responses, a simple procedure, a high success rate, and lower modeling costs, making it a more appropriate choice for constructing TEF models in rabbits.
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: World J Gastrointest Surg Año: 2024 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: World J Gastrointest Surg Año: 2024 Tipo del documento: Article País de afiliación: China
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