Allergenicity and Linear Epitope Analysis of Scy p 8, an Allergen from Mud Crab.
J Agric Food Chem
; 72(23): 13402-13414, 2024 Jun 12.
Article
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| MEDLINE
| ID: mdl-38821040
ABSTRACT
Scy p 8 (triosephosphate isomerase) as a crab allergen in inducing distinct T-helper (Th) cell differentiation and a linear epitope associated with allergenicity remain elusive. In this study, mice sensitized with Scy p 8 exhibited significantly upregulated levels of IgE, IgG1, and IL-4 release, inducing a Th2 immune response. Moreover, the release of IFN-γ (Th1) and the levels of Treg cells were downregulated, while IL-17A (Th17) was upregulated, indicating that Scy p 8 disrupted the Th1/Th2 balance and Th17/Treg balance in mice. Furthermore, bioinformatics prediction and serum samples from crab-allergic patients and mice enabled the discovery of 8 linear epitopes of Scy p 8. Meanwhile, the analysis of peptide similarity and tertiary superposition revealed that 8 epitopes of Scy p 8 exhibited conservation across various species, potentially resulting in cross-reactivity. These findings possess the potential to enhance the comprehension of crab allergens, thereby establishing a foundation for investigating cross-reactivity.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Alérgenos
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Braquiuros
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Ratones Endogámicos BALB C
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Epítopos
Límite:
Adult
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Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
J Agric Food Chem
Año:
2024
Tipo del documento:
Article