Bacterial Symbiont Mediates Aphid Antiviral Systems to Inhibit the Propagation of a New Pathogenic Densovirus in Chinese Wheat Aphid, Sitobion miscanthi.
J Agric Food Chem
; 2024 Jun 07.
Article
en En
| MEDLINE
| ID: mdl-38847422
ABSTRACT
Sitobion miscanthi L-type symbiont (SMLS) is a bacterial symbiont commonly found in the wheat aphid S. miscanthi. A new aphid densovirus, S. miscanthi densovirus (SmDV), was recently identified in S. miscanthi. In this study, the similar cellular tropism of SmDV and SMLS in aphid embryos was uncovered using in situ hybridization. SmDV infection significantly decreased the longevity and number of S. miscanthi offspring. However, the SmDV titers were significantly suppressed after SMLS transmission, thus reducing the negative effects of SmDV infection on S. miscanthi fitness. Moreover, an integrative analysis of RNA-seq datasets showed that SMLS inhibited the expression of genes related to the phosphatidylinositol 3-kinase (Pl3K)/Akt pathways and further induced the expression of antiviral factors associated with the apoptosis and FoxO signaling pathways. These results indicate that SMLS mediates host antiviral defenses to inhibit the propagation of SmDV, which was further verified by an RNA interference assay.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
J Agric Food Chem
/
J. agric. Food chem
/
Journal of agricultural and food chemistry
Año:
2024
Tipo del documento:
Article
País de afiliación:
China