Improving transient protein expression in agroinfiltrated Nicotiana benthamiana.
New Phytol
; 243(3): 846-850, 2024 Aug.
Article
en En
| MEDLINE
| ID: mdl-38849321
ABSTRACT
Agroinfiltration of Nicotiana benthamiana is routinely used in plant science and molecular pharming to transiently express proteins of interest. Here, we discuss four phenomena that should be avoided to improve transient expression. Immune responses can be avoided by depleting immune receptors and employing pathogen-derived effectors; transcript degradation by using silencing inhibitors or RNA interference machinery mutants; endoplasmic reticulum stress by co-expressing chaperones; and protein degradation can be avoided with subcellular targeting, protease mutants and co-expressing protease inhibitors. We summarise the reported increased yields for various recombinant proteins achieved with these approaches and highlight remaining challenges to further improve the efficiency of this versatile protein expression platform.
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Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Nicotiana
Idioma:
En
Revista:
New Phytol
Asunto de la revista:
BOTANICA
Año:
2024
Tipo del documento:
Article