Colorimetric-SERS dual-mode aptasensor for Staphylococcus aureus based on MnO2@AuNPs oxidase-like activity.
Food Chem
; 456: 139955, 2024 Oct 30.
Article
en En
| MEDLINE
| ID: mdl-38852453
ABSTRACT
The nanozyme-linked aptamer-sorbent assay (NLASA) is a rapid way to screen and characterize aptamer binding to targets. In this paper, a MnO2@AuNPs@aptamer (Apt) based NLASA coupled with colorimetric-SERS dual-mode for Staphylococcus aureus (S. aureus) detection is presented. Cu,Fe-CDs were used as the reducing agent to synthesize MnO2 and gold nanoparticles (AuNPs). Then, they were fabricated to obtain MnO2@AuNPs with oxidase (OXD)-like and SERS activities. The S. aureus aptamer was conjugated to MnO2@AuNPs and enhanced the OXD-like activity, which realized the specific capture of S. aureus in food matrices. In addition, S. aureus improves the oxidation of 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid (ABTS) but inhibits 3,3',5,5'-tetramethylbenzidine (TMB) to generate Raman-active oxTMB with MnO2@AuNPs@Apt. This sensor was used for detections of S. aureus in a concentration ranged from 101 to 107 CFU/mL with a detection limit of 0.926 CFU/mL (colorimetric) and 1.561 CFU/mL (SERS), and the recovery is 85%-105% in real samples.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Óxidos
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Oxidorreductasas
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Staphylococcus aureus
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Técnicas Biosensibles
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Compuestos de Manganeso
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Colorimetría
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Aptámeros de Nucleótidos
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Nanopartículas del Metal
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Oro
Idioma:
En
Revista:
Food Chem
Año:
2024
Tipo del documento:
Article