Application of hydrophobic eutectic solvent in efficient biotransformation of total flavonoids of Herba Epimedii.
J Biotechnol
; 391: 106-116, 2024 Aug 10.
Article
en En
| MEDLINE
| ID: mdl-38871028
ABSTRACT
Icaritin, a hydrolysate from total flavonoids of Epimedii (TFE), which has better anti-hepatoma activity than its glycosylated form. In this work, immobilized enzymes 4LP-Tpebgl3@Na-Y and DtRha@ES-107 were used to hydrolyze TFE to prepare icaritin. Five different hydrophobic deep eutectic solvents (HDES) were prepared and the most ideal HDES was successfully selected, which was composed of dodecyl alcohol and thymol with the molar ratio of 21. The relative enzyme activity of 4LP-Tpebgl3@Na-Y and DtRha@ES-107 was about 102.4â¯% and 112.5â¯%, respectively. In addition, the thermal and binding stability of 4LP-Tpebgl3@Na-Y and DtRha@ES-107 in HDES was not affected negatively. In the biphasic system composed of 50â¯% (v/v) HDES and Na2HPO4-citric acid buffer (50â¯mM, pH 5.5), 4LP-Tpebgl3@Na-Y (1.0â¯U/mL) and TFE (1â¯g/L) were reacted at 80⯰C for 1â¯h, and then reacted with DtRha@ES-107 (20â¯U/mL) at 80⯰C for 2â¯h. Finally, TFE was completely converted to 301.8â¯mg/L icaritin (0.82â¯mM). After 10 cycles, 4LP-Tpebgl3@Na-Y/DtRha@ES-107 still maintained 84.1â¯% original activity. In this study, we developed an efficient methodology for icaritin preparation through the integration of enzymatic catalysis and adsorption separation, presenting a viable approach for large-scale, cost-effective production of icaritin.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Flavonoides
/
Biotransformación
/
Enzimas Inmovilizadas
/
Interacciones Hidrofóbicas e Hidrofílicas
Idioma:
En
Revista:
J Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2024
Tipo del documento:
Article