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ß-asarone induces viability and angiogenesis and suppresses apoptosis of human vascular endothelial cells after ischemic stroke by upregulating vascular endothelial growth factor A.
Sun, Dazhong; Wu, Lulu; Lan, Siyuan; Chi, Xiangfeng; Wu, Zhibing.
Afiliación
  • Sun D; Department of Acupuncture and Moxibustion Rehabilitation, GuangDong Second Traditional Chinese Medicine Hospital, Guangzhou, China.
  • Wu L; The First School of Clinical Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China.
  • Lan S; School of Basic Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China.
  • Chi X; Department of Acupuncture and Moxibustion Rehabilitation, GuangDong Second Traditional Chinese Medicine Hospital, Guangzhou, China.
  • Wu Z; Department of Neurology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
PeerJ ; 12: e17534, 2024.
Article en En | MEDLINE | ID: mdl-38948219
ABSTRACT
Ischemic stroke (IS) is a disease with a high mortality and disability rate worldwide, and its incidence is increasing per year. Angiogenesis after IS improves blood supply to ischemic areas, accelerating neurological recovery. ß-asarone has been reported to exhibit a significant protective effect against hypoxia injury. The ability of ß-asarone to improve IS injury by inducing angiogenesis has not been distinctly clarified. The experimental rats were induced with middle cerebral artery occlusion (MCAO), and oxygen-glucose deprivation (OGD) model cells were constructed using human microvascular endothelial cell line (HMEC-1) cells. Cerebral infarction and pathological damage were first determined via triphenyl tetrazolium chloride (TTC) and hematoxylin and eosin (H&E) staining. Then, cell viability, apoptosis, and angiogenesis were assessed by utilizing cell counting kit-8 (CCK-8), flow cytometry, spheroid-based angiogenesis, and tube formation assays in OGD HMEC-1 cells. Besides, angiogenesis and other related proteins were identified with western blot. The study confirms that ß-asarone, like nimodipine, can ameliorate cerebral infarction and pathological damage. ß-asarone can also upregulate vascular endothelial growth factor A (VEGFA) and endothelial nitric oxide synthase (eNOS) and induce phosphorylation of p38. Besides, the study proves that ß-asarone can protect against IS injury by increasing the expression of VEGFA. In vitro experiments affirmed that ß-asarone can induce viability and suppress apoptosis in OGD-mediated HMEC-1 cells and promote angiogenesis of OGD HMEC-1 cells by upregulating VEGFA. This establishes the potential for ß-asarone to be a latent drug for IS therapy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación hacia Arriba / Supervivencia Celular / Apoptosis / Células Endoteliales / Factor A de Crecimiento Endotelial Vascular / Derivados de Alilbenceno / Accidente Cerebrovascular Isquémico / Anisoles Límite: Animals / Humans / Male Idioma: En Revista: PeerJ Año: 2024 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación hacia Arriba / Supervivencia Celular / Apoptosis / Células Endoteliales / Factor A de Crecimiento Endotelial Vascular / Derivados de Alilbenceno / Accidente Cerebrovascular Isquémico / Anisoles Límite: Animals / Humans / Male Idioma: En Revista: PeerJ Año: 2024 Tipo del documento: Article País de afiliación: China
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