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Silibinin improved the function of T cells in peripheral blood mononuclear cells (PBMCs) co-cultured with U-87 MG cell line.
Abadi, Banafshe; Abdesheikhi, Jahangir; Sedghy, Farnaz; Mahmoodi, Merat; Fallah, Hossein.
Afiliación
  • Abadi B; Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.
  • Abdesheikhi J; Brain Cancer Research Core, Universal Scientific Education and Research Network (USERN), Kerman, Iran.
  • Sedghy F; Equal first author.
  • Mahmoodi M; Department of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
  • Fallah H; Equal first author.
Avicenna J Phytomed ; 14(2): 166-176, 2024.
Article en En | MEDLINE | ID: mdl-38966629
ABSTRACT

Objective:

Silibinin has exhibited antitumor activities. However, there are few reports about the immunomodulatory properties of silibinin on T lymphocyte function in the tumor microenvironment. Here, we determined the effects of silibinin on T cells of peripheral blood mononuclear cells (PBMCs), cultivated alone or with a human cell line of glioblastoma (U-87 MG). Materials and

Methods:

The proliferation of T lymphocytes was assessed by MTT test in the presence of silibinin (15 and 45 µM). Also, total antioxidant capacity (TAC), the activity of superoxide dismutase-3 (SOD3), and the levels of two cytokines interferon gamma (IFN-γ) and tumor growth beta (TGF-ß) were compared between treated and untreated PBMCs alone or co-cultured with U-87 cells.

Results:

According to our results, silibinin raised the TAC levels and SOD3 activity in the PBMCs and in the co-culture condition. Moreover, silibinin-treated PBMCs showed higher IFN-γ levels and lower TGF-ß levels. Interestingly, silibinin protected PBMCs against the U-87-induced suppression.

Conclusion:

Altogether, these results proposed the immunomodulatory potential of silibinin on T cells of PBMCs, as well as its partially protective effects on PBMCs against the suppression induced by U-87 MG cells.
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Avicenna J Phytomed Año: 2024 Tipo del documento: Article País de afiliación: Irán

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Avicenna J Phytomed Año: 2024 Tipo del documento: Article País de afiliación: Irán
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