A covalent-organic framework-based platform for simultaneous smartphone detection and degradation of aflatoxin B1.

ABSTRACT

This study developed a smartphone-based biosensor that could simultaneously detect and degrade aflatoxin B1 (AFB1). A donor-acceptor covalent organic framework (COF) was bound onto the surface of stainless-steel mesh (SSM) via the in-situ synthesis, which was used to immobilize the aptamer (Apt) to specifically capture AFB1 and was also as a photocatalyst to degrade AFB1. Au@Ir nanospheres were synthesized, which exhibited better peroxidase catalytic activity (Km=5.36 × 10-6 M, Vmax=3.48 × 10-7 Ms-1, Kcat=1.00 × 107 s-1) than Ir@Au nanospheres, so Au@Ir nanospheres were linked with Apt2 to be utilized as the signal probe. The density functional theory calculation also described that Au@Ir nanospheres possessed the lower energy barriers to decompose H2O2 than Ir@Au nanospheres. Coupled with the "Color Picker" application in the smartphone, the established "sandwich-structure" colorimetric method exhibited a linear range of 0.5-200 µg L-1 and a detection limit of 0.045 µg L-1. The photocatalytic capacity of SSM/COF towards AFB1 was investigated and the degradation rate researched 81.14 % within 120 min under the xenon lamp irradiation, and the degradation products were validated by ESI-MS. It was applied for the detection of AFB1 in peanuts, corn, and wheat samples. Recoveries were ranging from 77.90 % to 112.5 %, and the matrix effect was 75.10-111.6 %. Therefore, the smartphone-based biosensor provided a simple, fast, and sensitive platform for the detection of AFB1, and meanwhile could realize the efficient degradation of AFB1.