Your browser doesn't support javascript.
loading
The intracellular C-terminus confers compartment-specific targeting of voltage-gated calcium channels.
Chin, Morven; Kaeser, Pascal S.
Afiliación
  • Chin M; Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA.
  • Kaeser PS; Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: kaeser@hms.harvard.edu.
Cell Rep ; 43(7): 114428, 2024 Jul 23.
Article en En | MEDLINE | ID: mdl-38996073
ABSTRACT
To achieve the functional polarization that underlies brain computation, neurons sort protein material into distinct compartments. Ion channel composition, for example, differs between axons and dendrites, but the molecular determinants for their polarized trafficking remain obscure. Here, we identify mechanisms that target voltage-gated Ca2+ channels (CaVs) to distinct subcellular compartments. In hippocampal neurons, CaV2s trigger neurotransmitter release at the presynaptic active zone, and CaV1s localize somatodendritically. After knockout of all three CaV2s, expression of CaV2.1, but not CaV1.3, restores neurotransmitter release. We find that chimeric CaV1.3s with CaV2.1 intracellular C-termini localize to the active zone, mediate synaptic vesicle exocytosis, and render release sensitive to CaV1 blockers. This dominant targeting function of the CaV2.1 C-terminus requires the first EF hand in its proximal segment, and replacement of the CaV2.1 C-terminus with that of CaV1.3 abolishes CaV2.1 active zone localization and function. We conclude that CaV intracellular C-termini mediate compartment-specific targeting.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hipocampo Límite: Animals / Humans Idioma: En Revista: Cell Rep Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hipocampo Límite: Animals / Humans Idioma: En Revista: Cell Rep Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos
...