Comparative assessment of the intragenomic variations of dinoflagellate Tripos species through single-cell sequencing.
Mar Pollut Bull
; 206: 116690, 2024 Sep.
Article
en En
| MEDLINE
| ID: mdl-39024906
ABSTRACT
Tripos is a large dinoflagellate genus widely distributed in the world's oceans. Morphology-based species identification is inconclusive due to high morphological intraspecific variability. Metabarcoding analysis has been demonstrated to be effective for species identification and tracking their spatiotemporal dynamics. However, accumulating evidence suggests high levels of intragenomic variations (IGVs) are common in many algae, leading to concerns about overinterpretation of molecular diversity in metabarcoding studies. In this project, we evaluated and compared IGVs in Tripos species by conducting the first high-throughput sequencing (HTS) of 18S rDNA V4 of Tripos single cells. High numbers of haplotypes (19-172) were identified in each of the 30 Tripos cells. Each cell contained one dominant haplotype with high relative abundance and many haplotypes with lower abundances. Thus, the presence of multiple minor haplotypes substantially overestimate the molecular diversity identified in metabarcoding analysis, which encompass not only interspecific and intraspecific diversities, but high levels of IGVs.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Dinoflagelados
/
Código de Barras del ADN Taxonómico
Idioma:
En
Revista:
Mar Pollut Bull
/
Mar. pollut. bull
/
Marine pollution bulletin
Año:
2024
Tipo del documento:
Article