Protocol for analysis of single-cell sequencing data by Seqtometry.
STAR Protoc
; 5(3): 103209, 2024 Aug 02.
Article
en En
| MEDLINE
| ID: mdl-39096493
ABSTRACT
Seqtometry (sequencing-to-measurement) is an analytical platform for single-cell analysis based on direct profiling of gene expression and accessibility achieved by advanced scoring with gene signatures. Here, we present a protocol for single-cell RNA sequencing (scRNA-seq) and single-cell assay for transposase-accessible chromatin using sequencing (scATAC-seq) analysis using Seqtometry. We describe steps for preprocessing, imputation, scoring, and plotting, with extensions to large datasets and integration of multiple datasets. This protocol yields results in the form of biologically interpretable dimensions for direct identification and comprehensive characterization of specific cells. For complete details on the use and execution of this protocol, please refer to Kousnetsov et al.1.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos