Multiplex PCR for gastrointestinal parasites in stool: Benchmarking against direct microscopy and simplex PCR.
Diagn Microbiol Infect Dis
; 110(2): 116475, 2024 Oct.
Article
en En
| MEDLINE
| ID: mdl-39096665
ABSTRACT
PURPOSE:
To develop and validate a multiplex conventional PCR assay to simultaneously detect Cryptosporidium spp., Entamoeba histolytica, and Giardia lamblia in diarrheal samples as a rapid, cost-effective, and sensitive diagnostic tool for prevalent co-infections for improved diagnostic accuracy and efficiency in resource-limited settings.METHODS:
Stool samples collected from patients with gastrointestinal symptoms after taking written consent, processed via wet mount, iodine mount, and PCR assays. Cohen's kappa statistical analysis was done to test agreement.RESULT:
Among 240 patients, 28.75% showed intestinal protozoa via Microscopy; Single-plex and multiplex PCR demonstrated 100% concordance, detecting 27.9%; confirmed by sequencing. Highest parasite positivity was observed in transplant and immunocompromised patients, with moderate to almost perfect agreement between microscopy and molecular methods.CONCLUSION:
Multiplex-conventional PCR offers superior sensitivity and specificity over microscopy and 100% concordance with single-plex PCR, enabling rapid, cost-effective diagnosis of multiple parasites from single stool sample. Its adoption could revolutionize parasitic infection management in routine diagnostics.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sensibilidad y Especificidad
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Giardia lamblia
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Entamoeba histolytica
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Heces
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Reacción en Cadena de la Polimerasa Multiplex
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Microscopía
Límite:
Adolescent
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Adult
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Aged
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
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Middle aged
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
/
Diagn. microbiol. infect. dis
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Diagnostic microbiology and infectious disease
Año:
2024
Tipo del documento:
Article
País de afiliación:
India