A microfluidic impedance cytometry device for robust identification of H. pluvialis.

ABSTRACT

H. pluvialis contains rich oleic acid and astaxanthin, which have important applications in the fields of biodiesel and biomedicine. Detection of live H. pluvialis is the prerequisite to obtaining oleic acid and astaxanthin. For this purpose, we successfully developed a reliable microfluidic impedance cytometry for the identification of live H. pluvialis. Firstly, we established a simulation model for detecting H. pluvialis based on their morphology and studied the effect of medium conductivity on the impedance of H. pluvialis at different frequencies. From the simulations, we determined that the optimal solution conductivity for the detection of H. pluvialis was 1500 µS cm-1 and studied the frequency responses of the impedance of H. pluvialis. Secondly, we fabricated the microchannels and stainless-steel detection electrodes and assembled them into microfluidic impedance cytometry. The frequency dependence of live and dead H. pluvialis was explored under different frequencies, and live and dead H. pluvialis were distinguished at a frequency of 1 MHz. The impedance of live H. pluvialis at the frequency of 1 MHz ranges from 33.73 to 52.23 Ω, while that of dead ones ranges from 13.05 to 19.59 Ω. Based on these findings, we accomplished the identification and counting of live H. pluvialis in the live and dead sample solutions. Furthermore, we accomplished the identification and counting of live H. pluvialis in the mixed samples containing Euglena and H. pluvialis. This approach possesses the promising capacity to serve as a robust tool in the identification of target microalgae, addressing a challenge in the fields of biodiesel and biomedicine.