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Fibronectin isoforms promote postnatal skeletal development.
Dinesh, Neha E H; Baratang, Nissan; Rosseau, Justine; Mohapatra, Ronit; Li, Ling; Mahalingam, Ramshaa; Tiedemann, Kerstin; Campeau, Philippe M; Reinhardt, Dieter P.
Afiliación
  • Dinesh NEH; Faculty of Medicine and Health Sciences, Department of Anatomy and Cell Biology, McGill University, Montreal, QC, Canada.
  • Baratang N; CHU Sainte-Justine Research Center, Montreal, QC, Canada.
  • Rosseau J; CHU Sainte-Justine Research Center, Montreal, QC, Canada.
  • Mohapatra R; Faculty of Medicine and Health Sciences, Department of Anatomy and Cell Biology, McGill University, Montreal, QC, Canada.
  • Li L; Faculty of Medicine and Health Sciences, Department of Anatomy and Cell Biology, McGill University, Montreal, QC, Canada.
  • Mahalingam R; Faculty of Dental Medicine and Oral Health Sciences, McGill University, Montreal, QC, Canada.
  • Tiedemann K; Shriners Hospitals for Children - Canada, Montréal, QC, Canada.
  • Campeau PM; CHU Sainte-Justine Research Center, Montreal, QC, Canada.
  • Reinhardt DP; Faculty of Medicine and Health Sciences, Department of Anatomy and Cell Biology, McGill University, Montreal, QC, Canada; Faculty of Dental Medicine and Oral Health Sciences, McGill University, Montreal, QC, Canada. Electronic address: dieter.reinhardt@mcgill.ca.
Matrix Biol ; 133: 86-102, 2024 Nov.
Article en En | MEDLINE | ID: mdl-39159790
ABSTRACT
Fibronectin (FN) is a ubiquitous extracellular matrix glycoprotein essential for the development of various tissues. Mutations in FN cause a unique form of spondylometaphyseal dysplasia, emphasizing its importance in cartilage and bone development. However, the relevance and functional role of FN during skeletal development has remained elusive. To address these aspects, we have generated conditional knockout mouse models targeting the cellular FN isoform in cartilage (cFNKO), the plasma FN isoform in hepatocytes (pFNKO), and both isoforms together in a double knockout (FNdKO). We used these mice to determine the relevance of the two principal FN isoforms in skeletal development from postnatal day one to the adult stage at two months. We identified a distinct topological FN deposition pattern in the mouse limb during different gestational and postnatal skeletal development phases, with prominent levels at the resting and hypertrophic chondrocyte zones and in the trabecular bone. Cartilage-specific cFN emerged as the predominant isoform in the growth plate, whereas circulating pFN remained excluded from the growth plate and confined to the primary and secondary ossification centers. Deleting either isoform independently (cFNKO or pFNKO) yielded only relatively subtle changes in the analyzed skeletal parameters. However, the double knockout of cFN in the growth plate and pFN in the circulation of the FNdKO mice significantly reduced postnatal body weight, body length, and bone length. Micro-CT analysis of the adult bone microarchitecture in FNdKO mice exposed substantial reductions in trabecular bone parameters and bone mineral density. The mice also showed elevated bone marrow adiposity. Analysis of chondrogenesis in FNdKO mice demonstrated changes in the resting, proliferating and hypertrophic growth plate zones, consistent alterations in chondrogenic markers such as collagen type II and X, decreased apoptosis of hypertrophic chondrocytes, and downregulation of bone formation markers. Transforming growth factor-ß1 and downstream phospho-AKT levels were significantly lower in the FNdKO than in the control mice, revealing a crucial FN-mediated regulatory pathway in chondrogenesis and bone formation. In conclusion, the data demonstrate that FN is essential for chondrogenesis and bone development. Even though cFN and pFN act in different regions of the bone, both FN isoforms are required for the regulation of chondrogenesis, cartilage maturation, trabecular bone formation, and overall skeletal growth.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Desarrollo Óseo / Fibronectinas / Ratones Noqueados / Isoformas de Proteínas / Placa de Crecimiento Límite: Animals Idioma: En Revista: Matrix Biol Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Canadá

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Desarrollo Óseo / Fibronectinas / Ratones Noqueados / Isoformas de Proteínas / Placa de Crecimiento Límite: Animals Idioma: En Revista: Matrix Biol Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Canadá
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