Glycerol-blended chitosan membranes with directional micro-grooves and reduced stiffness improve Schwann cell wound healing.
Biomed Mater
; 19(6)2024 Sep 10.
Article
en En
| MEDLINE
| ID: mdl-39208844
ABSTRACT
Regenerative medicine is continuously looking for new natural, biocompatible and possibly biodegradable materials, but also mechanically compliant. Chitosan is emerging as a promising FDA-approved biopolymer for tissue engineering, however, its exploitation in regenerative devices is limited by its brittleness and can be further improved, for example by blending it with other materials or by tuning its superficial microstructure. Here, we developed membranes made of chitosan (Chi) and glycerol, by solvent casting, and micro-patterned them with directional geometries having different levels of axial symmetry. These membranes were characterized by light microscopies, atomic force microscopy (AFM), by thermal, mechanical and degradation assays, and also testedin vitroas scaffolds with Schwann cells (SCs). The glycerol-blended Chi membranes are optimized in terms of mechanical properties, and present a physiological-grade Young's modulus (≈0.7 MPa). The directional topographies are effective in directing cell polarization and migration and in particular are highly performant substrates for collective cell migration. Here, we demonstrate that a combination of a soft compliant biomaterial and a topographical micropatterning can improve the integration of these scaffolds with SCs, a fundamental step in the peripheral nerve regeneration process.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células de Schwann
/
Cicatrización de Heridas
/
Materiales Biocompatibles
/
Movimiento Celular
/
Ingeniería de Tejidos
/
Quitosano
/
Andamios del Tejido
/
Módulo de Elasticidad
/
Glicerol
/
Regeneración Nerviosa
Límite:
Animals
Idioma:
En
Revista:
Biomed Mater
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Italia