Purification of polyphenol oxidase from tea (Camellia sinensis) using three-phase partitioning with a green deep eutectic solvent.
Food Chem X
; 23: 101720, 2024 Oct 30.
Article
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| MEDLINE
| ID: mdl-39229611
ABSTRACT
In this study, tea polyphenol oxidase (PPO) was purified via three-phase partitioning (TPP) using a deep eutectic solvent (DES) instead of t-butanol. First, the properties of 13 types of synthesized DESs were characterized, and DES-7 (thymol/dodecanoic acid) was selected as the best alternative solvent. The process parameters were optimized using response surface methodology. The experimental results revealed that when the (NH4)2SO4 concentration, DES to crude extract ratio, extraction time, and pH were 41%, 0.51, 75 min, and 5.6, respectively, the recovery and purification fold of tea PPO were 78.44% and 8.26, respectively. SDS-PAGE and native-PAGE were used to analyze the PPO before and after purification of the TTP system, and the molecular weight and purification effect of PPO were detected. Moreover, the DES could be recovered and recycled. The results indicate an environmentally friendly and stable DES, and provide a reference for the large-scale application of TPP to extract PPO.
Ammonium sulfate (PubChem CID:6097028); Catechol (PubChem CID:289); Deep eutectic solvents; Glycerol (PubChem CID:753); Menthol (PubChem CID:1254); Polyphenol oxidase; Purification; Tea; Theaflavin (PubChem CID:4263901); Three-phase partitioning; Thymol (PubChem CID:6989); t-Butanol (PubChem CID:6386)
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Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Food Chem X
Año:
2024
Tipo del documento:
Article
País de afiliación:
China