HPLC METHOD FOR THE QUANTIFICATION OF SOME ACTIVE FLAVONOIDS IN ETHYL ACETATE EXTRACT OF LEAVES OF BUTEA MONOSPERMA LINN.

ABSTRACT

AIM: The aim of the present investigation is to study HPLC process to evaluate Some Active Flavonoids in Ethyl Acetate Extract of Leaves of Butea monosperma Linn. MATERIAL AND METHODS: Using a soxhlation device, the leaves of Butea monosperma Linn. were extracted in stages. Each powdered batch (500g) was extracted in stages with polarity-graded solvents such as petroleum ether (Pet. Et) (60-80º), chloroform (CHCl3), ethyl acetate (EtOAc) using a soxhlet extractor. Alkaloids, flavonoids, glycosides, tannins, phenols, and steroids, among other chemical families of components, were identified through qualitative phytochemical screenings of each extract. To make a 10 g/ml stock, standard phenolic markers like quercetin, rutin, catechin, gallic acid, and chlorogenic acid were dissolved in methanol. Phytoconstituents were separated and identified from extracts using various solvents and combinations of solvents, which were chosen after consulting the literature. RESULTS AND DISCUSSION: Preliminary phytochemical screening showed the revealed that the leaves contain steroid, triterpenoids, fatty acid and alkaloids. While the ethyl acetate extract found to contain therapeutically important phytoconstitutes such as steroids, triterpenoids, saponins, flavonoids, and tannins. Bioactive extracts of Butea monosperma were found to include flavonoids and phenolic substances. In ethyl acetate extract, various flavonoids and phenolic compounds were discovered. CONCLUSION: This is a preliminary report on the identification of phytochemical and HPLC evaluation of ethyl acetate extract of leaves of Butea monosperma Linn. and to unravel the mechanisms driving bioactive qualities and the existence of putative synergy among these substances, more research is needed on the isolation and characterization of individual Flavonoids or phenolic compounds.