Experimental demonstration of an antigenic relationship between Leptospira and equine cornea.
Vet Immunol Immunopathol
; 10(2-3): 215-24, 1985 Nov.
Article
en En
| MEDLINE
| ID: mdl-4082476
ABSTRACT
Horses inoculated with either equine cornea or killed Leptospira interrogans serovars pomona, tarassovi, icterohaemorrhagiae, wolffi and hardjo, developed corneal opacity and produced antibodies which made it possible to demonstrate partial antigenic identity between equine cornea and four of those serovars employed. These antibodies were isolated by means of immunoadsorptions, purified by ion-exchange chromatography (DEAE-Sephadex A-50) and run by immuno-electrophoresis in agar gel. Both antibodies, anti-equine cornea and anti-leptospira, showed that they corresponded to the IgGb subclass. They bound themselves to equine cornea in vivo and in vitro as was proved by immunofluorescence. This antigenic relationship may be in part responsible for pathogenesis of corneal opacity in leptospirosis of horses.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Córnea
/
Leptospira interrogans
/
Antígenos
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
Vet Immunol Immunopathol
Año:
1985
Tipo del documento:
Article