Proposed role of lactate in germination of hypochlorite-treated Clostridium botulinum spores.
Appl Environ Microbiol
; 45(4): 1369-73, 1983 Apr.
Article
en En
| MEDLINE
| ID: mdl-6305268
ABSTRACT
Clostridium botulinum 12885A spores treated with hypochlorite required added DL-calcium lactate for L-alanine germination. Lactate was the active component of calcium lactate. Equimolar concentrations of L-malate, but not of DL-propionate, could replace lactate, suggesting that the alpha-hydroxy acid structure is important. Neither lactate nor malate was an effective germinant for buffer-treated or hypochlorite-treated spores. If the L-alanine concentration was increased 100-fold (to 450 mM), the lactate germination requirement was overcome. The data suggest that the L-alanine germination sites were modified by hypochlorite so that a higher concentration of alanine was required for activity. Lactate appeared to be an activator of modified or non-hypochlorite-modified L-alanine germination sites.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Clostridium botulinum
/
Ácido Hipocloroso
/
Lactatos
Idioma:
En
Revista:
Appl Environ Microbiol
Año:
1983
Tipo del documento:
Article