Singlet oxygen luminescence spectra: a comparison of interferometer- and grating-based spectrometers.
Photochem Photobiol
; 61(4): 350-2, 1995 Apr.
Article
en En
| MEDLINE
| ID: mdl-7740077
The current trend in methodology for determining IR and near-IR absorption spectra is to employ interferometer-based instruments to replace the monochromator-based devices used heretofore. As a dispersion element, the interferometer offers major improvements in spectral resolution (Connes advantage), light throughput (Jacquinot advantage) and data acquisition through multiplexing (Felgett advantage). We have compared signal-to-noise (S/N) ratios of grating-based and interferometer-based instruments for making spectral determinations of near-IR luminescence. Our results show that under identical excitation and detector conditions the interferometer instrument easily outperforms the grating, giving a 10-fold improvement in S/N at high signal amplitude (A488nm = 0.97) and a 20-fold improvement when the signal amplitude is low (A488nm = 0.06). Although some spectral resolution is sacrificed when scan times on the Fourier transform (FT)IR are significantly shortened, the S/N ratio was found only to decrease by a factor of 2 for a 10-fold decrease in scan time. This adds to the advantages of the FTIR technique because the S/N will thus improve for the same total acquisition time.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oxígeno
/
Espectrofotometría Infrarroja
/
Interferometría
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
Photochem Photobiol
Año:
1995
Tipo del documento:
Article
País de afiliación:
Estados Unidos