Nucleotide sequence and expression of UDP-glucose dehydrogenase gene required for the synthesis of xanthan gum in Xanthomonas campestris.
Biochem Biophys Res Commun
; 207(1): 223-30, 1995 Feb 06.
Article
en En
| MEDLINE
| ID: mdl-7857269
ABSTRACT
Xanthomonas campestris pv. campestris, producing large amounts of exopolysaccharide xanthan gum, has a mucoid phenotype. Strain SD7 was a non-mucoid mutant deficient in UDP-glucose dehydrogenase. A DNA fragment able to complement the mutation of SD7 was cloned from the parental wild-type strain Xc11. Sequence analysis of the region required for the complementation revealed an open reading frame which could encode a polypeptide of 445 amino acids with a calculated molecular weight of 48,432, a size similar to that of the product produced by maxicell. The amino acid sequence had significant homology to that of the GDP-mannose dehydrogenase from Pseudomonas aeruginosa.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos Bacterianos
/
Uridina Difosfato Glucosa Deshidrogenasa
/
Xanthomonas campestris
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1995
Tipo del documento:
Article
País de afiliación:
China