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Novobiocin modulates colchicine sensitivity in parental and multidrug-resistant B16 melanoma cells.
Nordenberg, J; Kornfeld, J; Wasserman, L; Shafran, M; Halabe, E; Beery, E; Landau, O; Novogrodsky, A; Sidi, Y.
Afiliación
  • Nordenberg J; Endocrinology Laboratory, Beilinson Medical Center, Petah Tiqva, Israel.
J Cancer Res Clin Oncol ; 120(10): 599-604, 1994.
Article en En | MEDLINE | ID: mdl-7929531
The effect of the antibiotic agent novobiocin on the sensitivity of melanoma cells to colchicine and vinblastine was examined in drug-sensitive and drug-resistant B16 melanoma cells. A cell line COL/R was selected for colchicine resistance. The COL/R cell line (resistant to 80 ng/ml colchicine) was found to possess the multidrug-resistant (MDR) phenotype. The cells were shown to be cross-resistant to vinblastine and Adriamycin and to overexpress P glycoprotein. P glycoprotein activity was assessed by using the rhodamine 123 accumulation test. Rhodamine accumulation was markedly decreased in COL/R cells as compared to the parental B16 cells. Verapamil reversed drug resistance and increased rhodamine accumulation in COL/R cells. Novobiocin in combination with colchicine or vinblastine synergistically inhibited the proliferation of parental B16 cells. In COL/R cells, novobiocin markedly decreased colchicine resistance and increased rhodamine accumulation. These data show that novobiocin increases the sensitivity of both parental and MDR melanoma cells to microtubule-disrupting cytotoxic drugs.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vinblastina / Colchicina / Novobiocina / Resistencia a Múltiples Medicamentos Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: J Cancer Res Clin Oncol Año: 1994 Tipo del documento: Article País de afiliación: Israel
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vinblastina / Colchicina / Novobiocina / Resistencia a Múltiples Medicamentos Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: J Cancer Res Clin Oncol Año: 1994 Tipo del documento: Article País de afiliación: Israel
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