Isolation and characterisation of promoter regions from Streptococcus thermophilus.

Four promoter regions required for the expression of a promoterless antibiotic resistance gene (cat194) in Streptococcus thermophilus were isolated by random chromosomal cloning experiments. These were shown to be functional in vivo, and their sequences were determined. Each region expressed different amounts of Cat protein as determined by enzyme activities. One region, STP10, was found to contain the 5' coding region of the large ribosomal subunit protein L20.