Expression of functional protease and subviral particles by vaccinia virus containing equine infectious anaemia virus gag and 5' pol genes.
J Gen Virol
; 75 ( Pt 4): 895-900, 1994 Apr.
Article
en En
| MEDLINE
| ID: mdl-8151302
ABSTRACT
Cells infected with vaccinia viruses expressing the equine infectious anaemia virus (EIAV) gag gene (VGag) or gag plus the 5' pol encoding protease (VGag/PR) were evaluated with monoclonal antibody to a p26 capsid protein linear epitope (QEISKFLTD). Both recombinant viruses expressed Gag precursor protein (55K) whereas only VGag/PR expressed a detectable Gag-Pol fusion protein (82K) with a functional protease, shown by subviral particles containing processed p26. Horses inoculated with VGag/PR produced antibodies reactive with EIAV Gag proteins.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Endopeptidasas
/
Virus Vaccinia
/
Proteínas de Fusión gag-pol
/
Virus de la Anemia Infecciosa Equina
Límite:
Animals
Idioma:
En
Revista:
J Gen Virol
Año:
1994
Tipo del documento:
Article