Overexpression and simple purification of a truncated, immunologically reactive GST-HCV core (1-123) fusion protein.
J Virol Methods
; 59(1-2): 13-21, 1996 May.
Article
en En
| MEDLINE
| ID: mdl-8793826
ABSTRACT
A full-length and a truncated gene for the core protein of hepatitis C virus (HCV) were linked to the gene for glutathione S-transferase (GST), and the expression of each GST-HCV core fusion protein was analyzed. The truncated GST-HCV core (1-123) fusion protein was expressed as a mostly soluble and partly insoluble form comprising more than 50% of the total protein in Escherichia coli after induction by isopropylthio-beta-D-galactoside (IPTG), while the full length GST-HCV core (1-191) fusion protein was not expressed, suggesting that the hydrophobic carboxy terminal region in the core protein affects its expression. In addition, the GST-HCV core (1-123) fusion protein purified by GST-agarose chromatography reacted specifically with an anti-HCV serum from a patient.
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
2_ODS3
Problema de salud:
2_enfermedades_transmissibles
Asunto principal:
Proteínas del Núcleo Viral
/
Hepatitis C
/
Hepacivirus
/
Antígenos de la Hepatitis C
Límite:
Humans
Idioma:
En
Revista:
J Virol Methods
Año:
1996
Tipo del documento:
Article