An evaluation of new gel matrices for the separation of PCR-amplified DNA fragments.
Mol Biotechnol
; 5(1): 63-5, 1996 Feb.
Article
en En
| MEDLINE
| ID: mdl-8853017
ABSTRACT
It has been shown that the novel gel matrix, PCR Purity Plus which consists of a vinyl-polymer of polyacrylamide, provides a superior and rapid means of separating DNA fragments. As this product has been discontinued, the two new commercial versions of this matrix (MDE and GeneAmp) with PCR Purity Plus were compared. Optimal conditions for resolving DNA fingerprint profiles for both matrices were defined. Both MDE and GeneAmp gels provided a clear separation of DNA fragments. However, the profiles obtained on GeneAmp gel were closest to that of PCR Purity Plus. These results should be useful to DNA fingerprinting studies where it is critical to obtain a clear resolution of complex DNA profiles.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Electroforesis
/
Geles
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
Mol Biotechnol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOTECNOLOGIA
Año:
1996
Tipo del documento:
Article
País de afiliación:
Estados Unidos