Onchocerca fasciata: histochemical demonstration of succinate and NADH dehydrogenase.
J Helminthol
; 70(1): 47-51, 1996 Mar.
Article
en En
| MEDLINE
| ID: mdl-8960199
ABSTRACT
The activities of selected enzymes of the respiratory chain system in Onchocerca fasciata (Filarioidea Onchocercidae) have been investigated histochemically. Thus, the localization and distributions of NADH dehydrogenase (EC 1.6.99.3), succinate dehydrogenase (SDH) (EC 1.3.99.1) and cytochrome oxidase (EC 1.9.3.1) were investigated in various tissues of the adult female worm by employing MTT, Nitro BT (dehydrogenases) and DAB (cytochrome oxidase). Different tissues varied considerably in their enzymatic activities. The hypodermis and reproductive tissues showed strong and identical localization of NADH and SDH dehydrogenase activities reflecting high metabolic rates. Little or no dehydrogenase activities were observed in the intestine and cuticle. In contrast to the two dehyrogenases, no activity was observed for cytochrome oxidase in any of the tissues in adult or embryonic stages of the worm. The significance of these results with respect to the energy metabolism of the worm is discussed. It is suggested that O. fasciata lacks a classical, mammalian-type respiratory pathway and that oxidative phosphorylation is of no importance as an energy generating pathway in this essentially anaerobic parasite.
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_zoonosis
Asunto principal:
Onchocerca
/
Oncocercosis
/
Succinato Deshidrogenasa
/
Camelus
/
NADH Deshidrogenasa
Límite:
Animals
Idioma:
En
Revista:
J Helminthol
Año:
1996
Tipo del documento:
Article
País de afiliación:
Arabia Saudita