Effects of food deprivation on the GH axis: immunocytochemical and molecular analysis.
Neuroendocrinology
; 65(2): 129-35, 1997 Feb.
Article
en En
| MEDLINE
| ID: mdl-9067990
ABSTRACT
Neuroendocrine mechanisms governing growth hormone (GH) secretion are sensitive to nutritional status since the normal pulsatile pattern of GH release is disrupted during conditions of food deprivation or malnutrition. A reasonable hypothesis for this occurrence is the alteration of somatostatin and GH-releasing hormone (GHRH) synthesis, storage and secretion. In this study, we investigated the effects of food deprivation on GH, GHRH, hypothalamic and pituitary galanin (GAL), and somatostatin through immunocytochemical and mRNA analysis. Adult male rats were subjected to 72 h of food deprivation, during which an average of 18% total body weight was lost. ICC studies were performed on brain sections from the rostral, middle and caudal regions of the median eminence of the hypothalamus using the avidin-biotin-peroxidase method. Immunocytochemical results were generated for the percent area and optical density (intensity) of immunostaining in the median eminence. Messenger RNA analyses were performed using sense and antisense riboprobes produced from cDNA clones for GH, GHRH, somatostatin and GAL. Food deprivation decreased somatostatin immunostaining in middle and caudal regions of the median eminence; similarly, food deprivation resulted in decreased GHRH immunostaining in rostral and middle sections of the median eminence of the hypothalamus. mRNA levels for somatostatin, GHRH and GH and GAL were also reduced by food deprivation. Our data suggest that suppressed GH secretion in food-deprived rats may reflect a general downregulation of the neuroendocrine and pituitary GH axis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hipófisis
/
Somatostatina
/
Hormona del Crecimiento
/
Hormona Liberadora de Hormona del Crecimiento
/
Privación de Alimentos
/
Hipotálamo
Límite:
Animals
Idioma:
En
Revista:
Neuroendocrinology
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos