Fructan of the inulin neoseries is synthesized in transgenic chicory plants (Cichorium intybus L.) harbouring onion (Allium cepa L.) fructan:fructan 6G-fructosyltransferase.
Plant J
; 11(3): 387-98, 1997 Mar.
Article
en En
| MEDLINE
| ID: mdl-9107030
ABSTRACT
Fructan (polyfructosylsucrose) is an important storage carbohydrate in many plant families. fructanfructan 6G-fructosyltransferase (6G-FFT) is a key enzyme in the formation of the inulin neoseries, a type of fructan accumulated by members of the Liliales. We have cloned the 6G-FFT from onion by screening a cDNA library using barley sucrosefructan 6-fructosyltransferase (6-SFT) as a probe. The deduced amino acid sequence showed a high homology with plant invertases and 6-SFT. Incubation of protein extracts from transgenic tobacco plants with the trisaccharide 1-kestose and sucrose resulted in the formation of neokestose and fructans of the inulin neoseries with a degree of polymerization up to six. Introduction of the onion 6G-FFT into chicory resulted in the synthesis of fructan of the inulin neoseries, in addition to the synthesis of linear inulin.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Allium
/
Plantas Modificadas Genéticamente
/
Fructanos
/
Hexosiltransferasas
/
Inulina
Idioma:
En
Revista:
Plant J
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BOTANICA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Países Bajos