Hepatocyte growth factor/scatter factor activates the apoptosis signaling pathway by increasing caspase-3 activity in sarcoma 180 cells.
Biochem Biophys Res Commun
; 245(1): 211-5, 1998 Apr 07.
Article
en En
| MEDLINE
| ID: mdl-9535810
ABSTRACT
Hepatocyte growth factor, which is now known to be the same protein as scatter factor, induced oligonucleosomal fragmentation of nuclear DNA of Sarcoma 180 cells and increased the activity of caspase-3, a key component in control of the apoptotic cell death pathway to about 2.6 times that in control cells on 48 hr incubation, but did not increase the activity of caspase-1. Both HGF-induced DNA fragmentation and caspase-3 activity were completely inhibited by co-incubation with an inhibitor of caspase-3, Ac-DEVD-H. In contrast, HGF did not affect the expression of the apoptosis suppressors Bcl-2 and Bcl-x. These results indicate that HGF activates the apoptosis signaling pathway by increasing caspase-3 activity in Sarcoma 180 cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sarcoma
/
Cisteína Endopeptidasas
/
Transducción de Señal
/
Factor de Crecimiento de Hepatocito
/
Apoptosis
/
Caspasas
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1998
Tipo del documento:
Article
País de afiliación:
Japón