Reconstitution of severe combined immunodeficient mice with spleen cells from autoimmune NZBxNZW F1 mice.
Clin Exp Rheumatol
; 16(1): 33-7, 1998.
Article
en En
| MEDLINE
| ID: mdl-9543559
ABSTRACT
OBJECTIVE:
The aim of this study is to establish an animal model to investigate the role of individual subsets of immune cells in the pathogenesis of systemic lupes erythematosus.METHODS:
Spleen cells isolated with from both young and old autoimmune NZB/W F1 mice were injected into the peritoneal cavity of severe combined immunodeficient (SCID) mice. Sera anti-DNA antibody levels and proteinuria of these SCID mice were followed regularly. In addition, histological changes of the kidneys were also examined.RESULTS:
The data suggest that anti-ss, dsDNA antibody can be detected in the sera of SCID mice 21 days after reconstitution with the spleen cells of either young or old NZB/W F1 mice, with titers of antibody increasing over time. In addition, proteinuria was also noted in most of these mice 3 months after reconstitution. Histopathological examination of the kidney also revealed the typical changes of glomerulonephritis. Immunofluorescence staining of kidney sections also demonstrated immune complex deposition.CONCLUSION:
Once validated, this animal model could be used in future studies to investigate the role of individual subsets of cells in the pathogenic mechanisms of SLE.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bazo
/
Ratones SCID
/
Lupus Eritematoso Sistémico
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Clin Exp Rheumatol
Año:
1998
Tipo del documento:
Article