Cloning of the ribosomal protein L41 gene of Phaffia rhodozyma and its use a drug resistance marker for transformation.
Appl Environ Microbiol
; 64(5): 1947-9, 1998 May.
Article
en En
| MEDLINE
| ID: mdl-9572978
ABSTRACT
The ribosomal protein L41 gene of Phaffia rhodozyma was cloned and used as a dominant selectable marker for cycloheximide resistance in the transformation of P. rhodozyma. Electrotransformation with a plasmid containing a ribosomal DNA fragment as a targeting signal typically yielded 800 to 1,200 transformants/microgram of DNA with an integrated copy number of about seven per haploid genome.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Ribosómicas
/
Transformación Genética
/
Levaduras
/
Cicloheximida
/
Genes Fúngicos
Idioma:
En
Revista:
Appl Environ Microbiol
Año:
1998
Tipo del documento:
Article