Differentiation of pathogenic and non-pathogenic leptospires by means of the polymerase chain reaction.
Rev Inst Med Trop Sao Paulo
; 39(4): 203-7, 1997.
Article
en En
| MEDLINE
| ID: mdl-9640782
ABSTRACT
A polymerase chain reaction was carried out to detect pathogenic leptospires isolated from animals and humans in Argentina. A double set of primers (G1/G2, B64-I/B64-II), described before, were used to amplify by PCR a DNA fragment from serogroups belonging to Leptospira interrogans but did not allow to detect saprophytic strains isolated from soil and water (L. biflexa). This fact represents an advantage since it makes possible the differentiation of pathogenic from non-pathogenic leptospires in cultures. The sensitivity of this assay has been determined, allowing to detect just only 10 leptospires in the reaction tube. Those sets of primers generated either a 285 bp or 360 bp fragment, depending on the pathogenic strain.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Reacción en Cadena de la Polimerasa
/
Leptospira
Límite:
Animals
/
Humans
País/Región como asunto:
America do sul
/
Argentina
Idioma:
En
Revista:
Rev Inst Med Trop Sao Paulo
Año:
1997
Tipo del documento:
Article
País de afiliación:
Argentina