Cysteine proteases of Trichomonas vaginalis degrade secretory leukocyte protease inhibitor.
J Infect Dis
; 178(3): 815-9, 1998 Sep.
Article
en En
| MEDLINE
| ID: mdl-9728551
ABSTRACT
Sexually transmitted diseases, including trichomoniasis, are risk factors for acquisition of human immunodeficiency virus (HIV) infection. Enhancement mechanisms are unknown. Secretory leukocyte protease inhibitor (SLPI) from saliva appears to prevent transmission of HIV through inhibition of virus entry into monocytic cells in vitro. This study was undertaken to determine if secreted cysteine proteases of Trichomonas vaginalis degrade SLPI and render it nonfunctional. It was determined if SLPI levels were decreased in vaginal fluids from pregnant women infected with T. vaginalis. Isolated proteases were incubated with recombinant human SLPI, and the degradation was followed by Western analysis with SLPI antiserum. SLPI levels were measured by ELISA in vaginal fluids from women infected with T. vaginalis and uninfected controls. Cysteine proteases cleaved SLPI and rendered it nonfunctional. Median levels of SLPI from infected patients were 26% of those of controls (P <.005). The degradation of SLPI in association with trichomonal infection may increase the risk of HIV acquisition.
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Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
4_TD
Problema de salud:
4_trichomoniasis
Asunto principal:
Tricomoniasis
/
Trichomonas vaginalis
/
Cisteína Endopeptidasas
/
Proteínas
/
Inhibidores de Serina Proteinasa
/
Complicaciones Parasitarias del Embarazo
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
J Infect Dis
Año:
1998
Tipo del documento:
Article
País de afiliación:
Estados Unidos