A Multiplex-PCR approach to identification of the Brazilian intermediate hosts of Schistosoma mansoni
Mem. Inst. Oswaldo Cruz
; 97(suppl.1): 95-97, Oct. 2002. ilus
Article
em En
| LILACS
| ID: lil-325030
Biblioteca responsável:
BR1.1
ABSTRACT
Due to difficulties concerning morphological identification of planorbid snails of the genus Biomphalaria, and given a high variation of characters and in the organs with muscular tissue, we designed specific polymerase chain reaction (PCR) primers for Brazilian snail hosts of Schistosoma mansoni from available sequences of internal transcribed spacer 2 (ITS2) of the ribosomal RNA gene. From the previous sequencing of the ITS2 region, one primer was designed to anchor in the 5.8S conserved region and three other species-specific primers in the 28S region, flanking the ITS2 region. These four primers were simultaneously used in the same reaction (Multiplex-PCR), under high stringency conditions. Amplification of the ITS2 region of Biomphalaria snails produced distinct profiles (between 280 and 350 bp) for B. glabrata, B. tenagophila and B. straminea. The present study demonstrates that Multiplex-PCR of ITS2-DNAr showed to be a promising auxiliary tool for the morphological identification of Biomphalaria snails, the intermediate hosts of S. mansoni
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Contexto em Saúde:
3_ND
Problema de saúde:
3_neglected_diseases
/
3_zoonosis
Assunto principal:
Schistosoma mansoni
/
Biomphalaria
/
Reação em Cadeia da Polimerase
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
/
Mem. Inst. Oswaldo Cruz
/
Mem. Inst. Oswaldo Cruz (Online)
/
Memórias do Instituto Oswaldo Cruz (Impresso)
/
Proceedings of the Instituto Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2002
Tipo de documento:
Article
/
Congress and conference
País de afiliação:
Brasil
/
Estados Unidos