Schistosoma mansoni antigen-driven interleukin-10 production in infected asthmatic individuals
Mem. Inst. Oswaldo Cruz
; 101(supl.1): 339-343, Oct. 2006. tab, graf
Article
em En
| LILACS
| ID: lil-441271
Biblioteca responsável:
BR1.1
ABSTRACT
Asthmatics infected with Schistosoma mansoni have a less severe course of asthma and an inhibition of the Th2 inflammatory response that seems to be mediated by interleukin (IL-10). The objective of this study was to evaluate the capacity of some S. mansoni antigens to stimulate IL-10 production in vitro by cells of asthmatic infected individuals. Peripheral bloods mononuclear cells were stimulated with the S. mansoni recombinant antigens Sm22.6, Sm14, P24, and PIII antigen. IL-10 was measured in the supernatants of cultures. As the recombinant antigens were cloned in Escherichia coli, we blocked contaminant endotoxin with polymyxin B added to the cultures. We demonstrated that all antigens used drove high production of IL-10 in S. mansoni infected individuals (n = 13, 408 ± 514 and 401 ± 383 pg/ml, 484 ± 245 pg/ml, 579 ± 468 pg/ml, respectively). In asthmatics infected with S. mansoni (n = 21) rP24 induced higher levels of IL-10 (565 ± 377 pg/ml) when compared to PIII, rSm14 and rSm22.6 (184 ± 209 pg/ml; 292 ± 243 pg/ml; 156 ± 247 pg/ml, respectively). Conclusion:
the S. mansoni antigens evaluated in this study stimulated IL-10 production by cells from infected individuals and therefore they have the potential to be used as a modulator of the inflammatory response in asthma.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Contexto em Saúde:
3_ND
Problema de saúde:
3_helminthiasis
/
3_neglected_diseases
/
3_schistosomiasis
/
3_zoonosis
Assunto principal:
Asma
/
Esquistossomose mansoni
/
Proteínas Recombinantes
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Antígenos de Helmintos
Limite:
Animals
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Female
/
Humans
/
Male
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2006
Tipo de documento:
Article
/
Project document
País de afiliação:
Brasil