Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
J. venom. anim. toxins incl. trop. dis
; J. venom. anim. toxins incl. trop. dis;13(4): 874-880, 2007. ilus
Article
em En
| LILACS
| ID: lil-471147
Biblioteca responsável:
BR33.1
ABSTRACT
A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Contexto em Saúde:
3_ND
Problema de saúde:
3_neglected_diseases
/
3_zoonosis
Assunto principal:
Plasmídeos
/
Vírus da Raiva
/
Proteínas Recombinantes de Fusão
/
Glicoproteínas
/
Transfecção
Idioma:
En
Revista:
J. venom. anim. toxins incl. trop. dis
Assunto da revista:
TOXICOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Brasil