[Identification and quantification of exogenous metabolites in biological liquids with new development in NMR spectroscopy in one and two dimensions]. / Identification et quantification de métabolites exogènes dans les liquides biologiques avec les nouveaux développements de la spectroscopie RMN en 1 et 2 dimensions.

ABSTRACT

By comparison of urine 1H NMR spectrum from a healthy person with those from poisoned patient, we have displayed one or several signals related to intoxication. In complement, various 1H and 13C NMR sequences in 1 and 2 dimensions (J-resolved, TOCSY, HMBC) were used to identify xenobiotic metabolites and quantify them in urine from patients poisoned with salicylate acetyl, chloroquine, valproïc acid, ethylene glycol and pesticides. NMR techniques provide a rapid determination on a small sample volume without pretreatment.